Anti-Cortactin (p80/85) Antibody, clone 4F11

Cortactin is present as a p80 and p85 kDa protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain. The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin. Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation. One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding.

80 kDa and 85 kDa

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src

Anti-Cortactin (p80/85) Antibody, clone 4F11 is an antibody against Cortactin (p80/85), and has been published and validated for use in IC, IH, IP and WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Recognizes Cortactin.

Format: Purified

Protein G Purified

Purified immunoglobulin in 10mM PBS, pH 7.4 with 0.05% sodium azide.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Positive Control Included: 3T3 Cell Lysate (12-305) Other controls: PC-12 cell lysate, HeLa whole cell lysate or A-431 whole cell lysate

routinely evaluated in mouse 3T3/A31 cell lysates or chicken fibroblast src-transformed cell lysate

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