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05-384

Anti-α-Actinin Antibody, clone AT6/172

Name: Anti-α-Actinin Antibody, clone AT6/172
Brand: MM

clone AT6/172, Upstate^®, from mouse

Synonym(s):

Anti-BDPLT15

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

AT6/172, monoclonal

Application:

ICC, WB

Citations:

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Properties

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AT6/172, monoclonal

species reactivity

rabbit, human, mouse

manufacturer/tradename

Upstate^®

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001102

UniProt accession no.

P12814

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACTN1(87)

Description

General description

100 kDa

Immunogen

Human α-actinin

Application

Immunoprecipitation is not recommended

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.

Biochem/physiol Actions

Recognizes α-actinin.

Physical form

Format: Purified

Please see product datasheet for lot-specific formulation.

Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated in immunoblot on human A431 cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-384:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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