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Merck

05-592

Anti-PP2A Antibody, B subunit, clone 2G9

ascites fluid, clone 2G9, Upstate®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2G9, monoclonal
Application:
IHC, IP, WB
Citations:
18

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2G9, monoclonal

species reactivity

rat, bovine, mouse, Xenopus, rabbit, pig, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NP_001009552

UniProt accession no.

P62714

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PPP2CA(5515)

General description

55kDa

Immunogen

Peptide corresponding to residues 398-411 of human PP2A, B subunit

Application

Detect PP2A using this Anti-PP2A Antibody, B subunit, clone 2G9 validated for use in IH, IP & WB.
Research Category
Signaling

Neuroscience
Research Sub Category
Kinases & Phosphatases

Neurodegenerative Diseases

Biochem/physiol Actions

PP2A B subunit, isoform reactivity is α > δ,β > γ

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and glycerol to 30%
Ascites

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from non-stimulated A431 cells or mouse 3T3/A31 cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-592:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein phosphatase 2A regulates estrogen receptor alpha (ER) expression through modulation of ER mRNA stability.
Keen, JC; Zhou, Q; Park, BH; Pettit, C; Mack, KM; Blair, B; Brenner, K; Davidson, NE
The Journal of Biological Chemistry null
Protein phosphatase 2A subunit assembly: the catalytic subunit carboxy terminus is important for binding cellular B subunit but not polyomavirus middle tumor antigen.
Ogris, E, et al.
Oncogene, 15, 911-917 (1997)
Jennifer B Jackson et al.
Neoplasia (New York, N.Y.), 14(7), 585-599 (2012-08-21)
Heterotrimeric protein phosphatase 2A (PP2A) consists of catalytic C (PP2Ac), structural A, and regulatory B-type subunits, and its dysfunction has been linked to cancer. Reversible methylation of PP2Ac by leucine carboxyl methyltransferase 1 (LCMT-1) and protein phosphatase methylesterase 1 (PME-1)
Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
Williams, BC; Filter, JJ; Blake-Hodek, KA; Wadzinski, BE; Fuda, NJ; Shalloway, D; Goldberg, ML
eLife null
Lihua Y Marmorstein et al.
The Journal of biological chemistry, 277(34), 30591-30597 (2002-06-12)
Bestrophin is a 68-kDa basolateral plasma membrane protein expressed in retinal pigment epithelial cells (RPE). It is encoded by the VMD2 gene, which is mutated in Best macular dystrophy, a disease characterized by a depressed light peak in the electrooculogram.
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Global Trade Item Number

SKUGTIN
05-59204053252730375

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