Skip to Content
Merck

07-449

Anti-trimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Synonym(s):

H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

dry ice

target post-translational modification

trimethylation (Lys27)

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

Application

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.
Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

Biochem/physiol Actions

Broad species cross-reactivity expected.
Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

Physical form

Format: Purified
Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.

Analysis Note

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-449:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Su(z)2 antagonizes auto-repression of Myc in Drosophila, increasing Myc levels and subsequent trans-activation.
Khan, A; Shover, W; Goodliffe, JM
Testing null
A WD40 domain cyclophilin interacts with histone H3 and functions in gene repression and organogenesis in Arabidopsis.
Li, H; He, Z; Lu, G; Lee, SC; Alonso, J; Ecker, JR; Luan, S
Plant Cell null
COOH-terminal binding protein regulates expression of the p16INK4A tumor suppressor and senescence in primary human cells.
Mroz, EA; Baird, AH; Michaud, WA; Rocco, JW
Cancer Research null
An extended transcriptional network for pluripotency of embryonic stem cells.
Kim, J; Chu, J; Shen, X; Wang, J; Orkin, SH
Cell null
RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena.
Liu, Y; Taverna, SD; Muratore, TL; Shabanowitz, J; Hunt, DF; Allis, CD
Genes & Development null
View All Related Papers

Articles

Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

抗体とは：抗原・エピトープ・抗体

抗体は特定の抗原と結合し、ユニークな抗原－抗体複合体を形成します。この結合の性質や、強固かつ特異的なこの結合を分子的標識として研究に使用する方法について説明します。

抗体の産生 | モノクローナル抗体とポリクローナル抗体の比較

抗体がどのように産生されるのか、クローン数、抗体のフォーマットなど、モノクローナル抗体とポリクローナル抗体の違いを説明します。

免疫蛍光法プロトコルのコツ

免疫蛍光法では、タンパク質の局在化、修飾の確認、タンパク質複合体の可視化のために抗体結合蛍光分子を使用します。

See All

Protocols

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

免疫組織染色（IHC）のトラブルシューティング：うまくいくコツ

発色剤を用いた明視野解析と蛍光顕微鏡法を用いたFFPEおよび凍結組織を利用した免疫組織染色（IHC）プロトコルのトラブルシューティング：うまくいくコツ

Global Trade Item Number

SKUGTIN
07-44904053252671517

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service