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1.05230

Oil red O (C.I. 26125)

for microscopy Certistain®

Synonym(s):

Oil red O (C.I. 26125)

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About This Item

CAS Number:
1320-06-5
Molecular Weight:
408.5
EC Index Number:
215-295-3
UNSPSC Code:
41116130
MDL number:
MFCD00003898
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composition

Dye, ≥80% spectrophotometric assay

mp

182-186 °C

bulk density

270 kg/m3

εmax

725-910 at 528-534 nm in chloroform at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

General description

The dry dye Oil red O (C.I. 26125) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections. As a Certistain® dye it is chemically quality-certified according to highest standards and strict specifications. Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues, in joint pathology or in cytological sample material. This Oil red O solution is ready-to-use and results in a brilliant, bright red staining of lipids, while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Cat. no. 105175).
The staining of lipids in histology specimens is a physical process. Oil red O is a lipophilic dye; due to its solubility characteristics it diffuses to the tissue lipids. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Certistain® dry dye used to detect lipids in cryosections

Analysis Note

Dye content (spectrophotometrically)≥ 80 %Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (chloroform)528 - 534 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; chloroform)725 - 910TLC-Testpasses testLoss on drying (110 °C)≤ 8 %Suitability for microscopypasses test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

1052300000: + 1052300025: + 1052309999:

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