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1.08218

Trifluoroacetic acid solution

(25% solution in water) for protein sequenation

Synonym(s):

Trifluoroacetic acid solution, TFA solution, TFA

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About This Item

Linear Formula:
CF3COOH
CAS Number:
76-05-1
Molecular Weight:
114.02
NACRES:
NB.21
UNSPSC Code:
12352106
Beilstein/REAXYS Number:
5446934
MDL number:
MFCD00004169
Concentration:
24.5-25.5% (trifluoroacetic acid)
Form:
liquid
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Quality Segment

200

form

liquid

composition

Water, 74.5-75.5% Karl Fischer

concentration

24.5-25.5% (trifluoroacetic acid)

density

1.12 g/cm3 at 20 °C

storage temp.

15-25°C

SMILES string

OC(=O)C(F)(F)F

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

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pictograms

Corrosion
GHS05

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

wgk

WGK 2

hcodes

H314,H412

Storage Class

8B - Non-combustible corrosive hazardous materials

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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