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Merck

16-104

Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate

clone 4G10®, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
FITC conjugate
Clone:
4G10®, monoclonal
Application:
ICC
Citations:
12

Key Documents

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biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody product type

primary antibodies

clone

4G10®, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr)

Gene Information

human ... PID1(55022)

General description

Anti-phosphotyrosine antibody (05-321), cross-linked to fluorescein isothiocyanate (FITC).

Immunogen

Phosphotyramine-KLH.

Application

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, FITC conjugate validated for use in IC.
For use in applications in which a FITC conjugate is advantageous, such as ICC.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Biochem/physiol Actions

Recognizes tyrosine-phosphorylated proteins from all species.

Physical form

PBS containing 0.05% sodium azide
Protein G Purified

Preparation Note

Stable for 9 months at 4°C

Analysis Note

Control
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
routinely evaluated in immunocytochemistry to detect tyrosine phosphorylated proteins in EGF-stimulated human A431 cells

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

16-104:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaomin Zhang et al.
Neuron, 107(6), 1212-1225 (2020-08-09)
Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal CA3 region, but how they process spatial information remains enigmatic. To examine the role of GCs in spatial coding, we measured excitatory postsynaptic potentials (EPSPs) and action potentials
Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses.
Sestak, K; Feely, S; Fey, B; Dufour, J; Hargitt, E; Alvarez, X; Pahar, B; Gregoricus et al.
Testing null
Kelly L Asquith et al.
Journal of cell science, 117(Pt 16), 3645-3657 (2004-07-15)
Mammalian spermatozoa undergo a series of molecular and biochemical changes collectively termed capacitation prior to acquiring the ability to fertilise the oocyte. Although phosphorylation of sperm proteins on tyrosine residues has been recognised as an important component of this process
Carolina Borges-Merjane et al.
Neuron, 105(6), 992-1006 (2020-01-14)
How structural and functional properties of synapses relate to each other is a fundamental question in neuroscience. Electrophysiology has elucidated mechanisms of synaptic transmission, and electron microscopy (EM) has provided insight into morphological properties of synapses. Here we describe an
Stefano De Tito et al.
Methods in molecular biology (Clifton, N.J.), 2557, 225-234 (2022-12-14)
The Golgi apparatus is a highly dynamic organelle that controls lipid and protein sorting in the endocytic and exocytic cellular pathways. Perturbation of the lipid homeostasis or of the molecular machineries that regulate membrane remodeling/trafficking events on the Golgi membranes
View All Related Papers

Global Trade Item Number

SKUGTIN
16-10404053252473159
SCC07204055977179606

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