Anti-CD28 Mouse mAb (ANC28.1/5D10)

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.

Recognizes CD28 on human T cells and BHK cells transfected with human CD28.

Human

human CD28 Ig fusion protein and HPB-MLT human tumor cells

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see comments)

Please refer to vial label for lot-specific concentration.

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.

Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x10⁵ cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)