Skip to Content
Merck

Key Documents

View All Documentation

382065

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride

Cell-permeable, adenine-thymine-specific fluorescent stain.

Synonym(s):

Bisbenzimide H 33342 Fluorochrome, Trihydrochloride, 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C27H28N6O · 3HCl
CAS Number:
875756-97-1
Molecular Weight:
561.93
MDL number:
MFCD00166996
UNSPSC Code:
12352111
NACRES:
NA.21
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

100

description

RTECS - DT4200000

assay

≥95% (TLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

yellow

solubility

water: 50 mg/mL

fluorescence

λex 346 nm, λem 460 nm

shipped in

ambient

storage temp.

2-8°C

SMILES string

N1(CCN(CC1)c2cc3nc([nH]c3cc2)c4cc5nc([nH]c5cc4)c6ccc(cc6)OCC)C

InChI

1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)

InChI key

PRDFBSVERLRRMY-UHFFFAOYSA-N

General description

Cell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.
Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Adenine-thymine-specific fluorescent stain
Product does not compete with ATP.
Reversible: no

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol.118, 148.
Sandhu, L.C., et al. 1985. Cytometry6, 191.
Frankfurt, O.S. 1983. Cytometry4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA78, 363.
Visser, J.W.M. 1980. Flow CytometryIV, 86.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

382065-100MG: + 382065-MG:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Need A Sample COA?

This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library