48-621MAG

MILLIPLEX^® DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay

Name: MILLIPLEX<SUP>®</SUP> DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay
Brand: MM

Synonym(s):

Cell Signaling DNA Damage Kit, DNA Damage Bead Panel, Genotoxicity Magnetic Beads

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About This Item

UNSPSC Code:

12161503

NACRES:

NA.47

Key Documents

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species reactivity

mouse, human, rat

Quality Level

200

manufacturer/tradename

Milliplex^®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex^® xMAP^®)

storage temp.

2-8°C

Related Categories

Immunoassay Platform Solutions

MILLIPLEX^® Multiplex Assays for Luminex^® Instruments

General description

The MILLIPLEX^® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates:• ATR (total)• Chk1 (Ser345)• Chk2 (Thr68)• H2A.X (Ser139)• MDM2 (total)• p21 (total)• p53 (Ser15)

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 1 (provided).The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:ATR (Total);Chk1 (Ser345);Chk2 (Thr68);H2A.X (Ser139);MDM2 (total);p21 (Total);p53 (Ser15)

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

GHS05,GHS07,GHS09

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Precautionary Statements

P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.