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Merck

524625

Millipore

Phosphatase Inhibitor Cocktail II

liquid, for the inhibition of acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTP's). Suitable for use with cell lysates and tissue extracts.

Synonym(s):

Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

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Product Name

Phosphatase Inhibitor Cocktail Set II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.

Quality Level

100

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.

Application

Phosphatase Inhibitor Cocktail Set II has been used:
  • in triple detergent buffer to lyse cultured cells to extract total protein for western blots
  • as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins
  • as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2)
  • as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting

Biochem/physiol Actions

Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.

Features and Benefits

  • Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
  • Economical: ready-to-use format minimizes wastage.
  • Effective: one ml is adequate for approximately 100 ml extraction buffer.
  • Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Provided as a set of five 1 ml vials containing the following components:
  • Imidazole (200 mM)
  • Sodium Fluoride (100 mM)
  • Sodium Molybdate (115 mM)
  • Sodium Orthovanadate (100 mM)
  • Sodium Tartrate Dihydrate (400 mM)      

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

524625-1.0ML:
524625-1EA:
524625-10ML:
524625-100ML:
524625-1SET:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rui Dong et al.
Journal of neuroinflammation, 19(1), 64-64 (2022-03-09)
Postoperative cognitive dysfunction (POCD) is a common complication following anesthesia and surgery. General anesthetic isoflurane has potential neurotoxicity and induces cognitive impairments, but the exact mechanism remains unclear. Astrocytes form interconnected networks in the adult brain through gap junctions (GJs)
Yoshitaka Ohno et al.
Cells, tissues, organs, 194(5), 363-370 (2011-03-23)
Heat stress is one of the hypertrophic stimuli on mammalian skeletal muscle. Nuclear factor-κB (NF-κB) signaling plays an important role in the regulation of skeletal muscle mass. However, the effects of heat stress on NF-κB signaling in skeletal muscle cells
Igor Moura de Paiva et al.
Molecular pharmaceutics, 19(6), 1825-1838 (2022-03-11)
The disruption of polynucleotide kinase/phosphatase (PNKP) in colorectal cancer (CRC) cells deficient in phosphatase and tensin homolog (PTEN) is expected to lead to the loss of cell viability by a process known as synthetic lethality. In previous studies, we have
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Global Trade Item Number

SKUGTIN
524625-1SETCN07790788051068