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Merck

539134

Protease Inhibitor Cocktail III

EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.

Synonym(s):

EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

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Product Name

Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

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General description

Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.

Application

Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
  • as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
  • as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
  • in lysis buffer to extract proteins for immunoblotting

Biochem/physiol Actions

Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.
Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.

Features and Benefits

  • EDTA-Free: recommended for IMAC applications.
  • Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
  • Fast: no waiting time for tablets to dissolve.
  • Economical: ready-to-use format reduces wastage.
  • Effective: one ml is sufficient for approximately 20g of tissue.
  • Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - (refers to pure substance)

flash_point_c

87 °C - (refers to pure substance)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water soluble liquid

fsl

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

539134-100ML: + US1539134-1SET: + 539134-1UNML: + 539134-10ML: + US1539134-1ML: + 539134-25SUL: + 539134-1ML: + 539134-1SET: + US2539134-1ML: + 539134-1SML: + 539134-1EA: + 539134-50SML: + 539134-50ML:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniel S Levic et al.
The Journal of cell biology, 219(4) (2020-04-25)
Epithelial cell physiology critically depends on the asymmetric distribution of channels and transporters. However, the mechanisms targeting membrane proteins to the apical surface are still poorly understood. Here, we performed a visual forward genetic screen in the zebrafish intestine and
Olga Vasiljeva et al.
Scientific reports, 10(1), 5894-5894 (2020-04-05)
Proteases have been implicated in the development of many pathological conditions, including cancer. Detection of protease activity in diseased tissues could therefore be useful for diagnosis, prognosis, and the development of novel therapeutic approaches. Due to tight post-translational regulation, determination
Cao Chen et al.
Medical microbiology and immunology, 209(1), 81-94 (2019-11-14)
Activation of complement system in central nervous system (CNS) of the patients suffering from prion diseases or animal models infected with prion agents experimentally is reported repeatedly, but which pathways are involved in the complement system during prion infection is
Bruno E Rojas et al.
Journal of experimental botany, 72(7), 2514-2524 (2020-12-15)
Phosphoenolpyruvate carboxykinase (PEPCK) plays a crucial role in gluconeogenesis. In this work, we analyze the proteolysis of Arabidopsis thaliana PEPCK1 (AthPEPCK1) in germinating seedlings. We found that the amount of AthPEPCK1 protein peaks at 24-48 h post-imbibition. Concomitantly, we observed
Wei-Ling Wang et al.
American journal of physiology. Renal physiology, 318(4), F956-F970 (2020-02-25)
Aquaporin-2 (AQP2) is a vasopressin-regulated water channel protein responsible for osmotic water reabsorption by kidney collecting ducts. In response to vasopressin, AQP2 traffics from intracellular vesicles to the apical plasma membrane of collecting duct principal cells, where it increases water
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Articles

How to Use Inhibitors

Learn tips and tricks for how to properly use inhibitors including how to select the right inhibitor and how to plan experiments with inhibitors.

Global Trade Item Number

SKUGTIN
539134-1SETCN07790788059750
539134-1MLCN07790788051280

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