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Merck

71341

pRSFDuet-1 DNA - Novagen

Synonym(s):

pRSFDuet-1 Vector

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106602
Promoter:
Promoter name: T7

Key Documents

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form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

promoter

Promoter name: T7

selection

kanamycin

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

General description

pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.

Application

pRSFDuet-1 DNA - Novagen has been used as a vector:
  • to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression
  • to clone the coding region of Mad1597–719
  • to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

71341-3: + 71341-MG: + 71341-10UG:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network

Global Trade Item Number

SKUGTIN
71341-3CN04055977253283

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