AB5475
Anti-Prox 1 Antibody
serum, Chemicon®
Synonym(s):
Anti-A230003G05Rik, Anti-PROX-1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Prox 1 Antibody, serum, Chemicon®
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, zebrafish
species reactivity (predicted by homology)
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PROX1(5629)
Analysis Note
Control
Embryonic brain tissue.
Embryonic brain tissue.
Application
Detect Prox 1 using this Anti-Prox 1 Antibody validated for use in IC, IH.
Immunohistochemistry:
1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum.
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry
1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum.
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry
Biochem/physiol Actions
Other species have not been tested.
Recognizes Prox1. The antibody is useful for identifying Prox1 expressing cells during the development of many areas of the nervous system including the dorsal spinal cord, ganglionic eminences, thalamus and hippocampus.
General description
Prox1, the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1.
Immunogen
Synthetic peptide from the C-terminus of mouse Prox1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Rabbit serum in 50 % glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB5475 | 04053252625466 |
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