Skip to Content

Key Documents

View All Documentation

GF144

Epidermal Growth Factor Protein, Human recombinant

>98% (SDS-PAGE), recombinant, expressed in E. coli

Synonym(s):

EGF

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405
Assay:
>98% (SDS-PAGE)
Impurities:
<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Epidermal Growth Factor Protein, Human recombinant, The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.

Quality Segment

100

assay

>98% (SDS-PAGE)

specific activity

≥1 x 10^7 U/mg

manufacturer/tradename

Chemicon®

impurities

<0.1 ng/μg Endotoxin (of EGF; 1EU/μg)

input

sample type neural stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)

NCBI accession no.

NM_001963.2

UniProt accession no.

P01133

shipped in

dry ice

storage temp.

-10 to -25°C

General description

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Product Source: E.coli

Application

For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.

Physical form

Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.

Preparation Note

The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.

Analysis Note

Specific Activity: Human EGF is fully biologically active when compared to standards.The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL

Other Notes

Replaces: GF001

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

GF144:

jan

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library