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Merck

HSTCMAG-28SK

MILLIPLEX® Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Synonym(s):

Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Key Documents

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Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 98-107%
sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC)
standard curve range: 0.18-750 pg/mL
(IL-6)
standard curve range: 0.24-1,000 pg/mL
(IL-13)
standard curve range: 0.24-1,000 pg/mL
(IL-21)
standard curve range: 0.31-1,250 pg/mL
(IL-8)
standard curve range: 0.31-1,250 pg/mL
(MIP-1α)
standard curve range: 0.37-1,500 pg/mL
(IL-7)
standard curve range: 0.45-1,750 pg/mL
(TNFα)
standard curve range: 0.49-2,000 pg/mL
(IL-1β)
standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))
standard curve range: 0.49-2,000 pg/mL
(IL-2)
standard curve range: 0.49-2,000 pg/mL
(IL-5)
standard curve range: 0.61-2,500 pg/mL
(IFNγ)
standard curve range: 0.61-2,500 pg/mL
(MIP-3α)
standard curve range: 0.73-3,000 pg/mL
(IL-17A)
standard curve range: 0.92-3,750 pg/mL
(MIP-1β)
standard curve range: 1.22-5,000 pg/mL
(GM-CSF)
standard curve range: 1.46-6,000 pg/mL
(IL-10)
standard curve range: 1.46-6,000 pg/mL
(ITAC)
standard curve range: 1.83-7,500 pg/mL
(IL-4)
standard curve range: 18.3-75,000 pg/mL
(Fractalkine)
standard curve range: 7.93-32,500 pg/mL
(IL-23)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

The MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel is to be used for the simultaneous quantification of any or all of the following analytes in human plasma, serum, and cell/tissue culture supernatant samples: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α
and TNFα.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α
  • Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
  • Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience

Biochem/physiol Actions

Cross Reactivty
There is no detectable cross-reactivity within the panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.
Replaces: HSTCMAG-28SK; HSCYTMAG-60SK
Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yu-Fei Gao et al.
International journal of ophthalmology, 12(11), 1708-1713 (2019-11-20)
To compare the anti-inflammatory effects of intense pulsed light (IPL) with tobramycin/dexamethasone plus warm compress through clinical signs and cytokines in tears. Eighty-two patients with dry eye disease (DED) associated meibomian gland dysfunction (MGD) were divided into two groups. Group
Bruna M Ascoli et al.
International journal of bipolar disorders, 7(1), 13-13 (2019-06-04)
Innate immune system dysfunction has been recognized as an important element in the pathophysiology of bipolar disorder (BD). We aimed to investigate whether there are differences in the response of macrophages derived from patients in the early stages and late
B Karshikoff et al.
Brain, behavior, and immunity, 57, 38-46 (2016-04-09)
Low-grade systemic inflammation has been implicated in chronic pain, as well as in comorbid diseases like depression and fatigue. We have previously shown that women's pain perception and regulation is more affected by systemic inflammation than that of men. Here
Joshua Y Lee et al.
Canadian journal of pain = Revue canadienne de la douleur, 5(1), 30-42 (2021-05-15)
Background: The prevalence of inadequate treatments for chronic pain has necessitated the search for biological factors that influence the transition to chronicity. Methods: Antecubital blood was drawn from those who experienced acute, noncatastrophic musculoskeletal trauma. Follow-up occurred at 1, 3
Amanda Priscila de Oliveira et al.
Cytokine, 91, 51-56 (2016-12-22)
The aim of this study was to investigate the plasma levels of the CCL3 and CCL4 chemokines in patients with the cardiac and digestive clinical forms of chronic Chagas disease and in cardiac patients with and without left ventricular systolic
View All Related Papers

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Immunology multiplex assays, like MILLIPLEX® assays, allow researchers to investigate more immune biomarkers simultaneously, saving time and resources.

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