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HX0514

Hyaluronidase

Synonym(s):

Hyaluronidase

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About This Item

CAS Number:
37326-33-3
UNSPSC Code:
12352202
NACRES:
NA.78
MDL number:
MFCD00131351
Specific activity:
≥300 units/mg dry wt.
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conjugate

conjugate (Glucosaminoglycan)

Quality Level

100

form

powder

specific activity

≥300 units/mg dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

shipped in

ambient

storage temp.

−20°C

General description

300 units/mg minimum, 50,000 units/vial. Suitable for biochemistry grade.

Specifications:Activity: 300NF U/mg min





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Hyaluronidase has been used:
  • to enzymatically remove extra-cellular matrix (ECM) to assess the lateral diffusion of the synaptic a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)
  • to study the effects of 4-methylumbelliferone (4MU)/ hyaluronidase (HLX)-mediated depletion of the CD44/hyaluronan complex on trastuzumab binding in JIMT1 breast cancer xenografts
  • as a component of Hank′s balanced salt solution (HBSS) medium to digest fetal intestine tissue

Biochem/physiol Actions

Hyaluronidase is an endoglycosidase that degrades hyaluronic acid (HA), a component of the extracellular matrix. It is used to enhance drug diffusion and reverse the effects of HA filler injections. Hyaluronidases dissolve the subcutaneous nodules and can correct excessive quantities of injected filler. It eliminates the cumulus-corona-oocyte complex formed during intracytoplasmic sperm injection. Hyaluronidase inhibits tissue damage after the extravasation of several substances. It is used for reducing edema treating vitreous hemorrhage.

Physical form

Dialyzed, lyophilized powder, not less than300 units per mg (dry weight).

Other Notes

One unit is based on the change in absorbency (turbidity) at 540 nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

HX0514-1:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Maurizio Cavallini et al.
Aesthetic surgery journal, 33(8), 1167-1174 (2013-11-08)
Hyaluronidases, a family of enzymes that are able to degrade hyaluronic acid (HA), are employed in medicine to increase drug diffusion and reverse the effects of HA filler injections. Hyaluronidases are able to dissolve subcutaneous nodules or to correct excessive
Sang Hak Lee et al.
eLife, 6 (2017-07-28)
Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR trafficking outside of, and within, post-synaptic densities (PSDs) from rats. Organic fluorescent
Patricia M R Pereira et al.
Molecular pharmaceutics, 17(1), 327-337 (2019-12-06)
Human epidermal growth factor receptor 2 (HER2) is a biomarker in breast cancer, and its overexpression is required to initiate therapies using HER2-targeted antibodies. Although trastuzumab is one of the most effective therapeutic antibodies in HER2-overexpressing breast cancer, a significant

Global Trade Item Number

SKUGTIN
HX0514-1CN04055977230291