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Merck

MAB318

Anti-Tyrosine Hydroxylase Antibody

CHEMICON®, mouse monoclonal, LNC1

Synonym(s):

TH, Tyrosine Monooxygenase, Tyrosine 3-monooxygenase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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Product Name

Anti-Tyrosine Hydroxylase Antibody, clone LNC1, ascites fluid, clone LNC1, Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LNC1, monoclonal

species reactivity

zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_000360.3

UniProt accession no.

P07101

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TH(7054)
rat ... Th(25085)

Analysis Note

Control
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Routinely evaluated by Western Blot on Mouse Brain lysates.

Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.

Application

Detect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
Immunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).

Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

59-63 kDa
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Immunogen

Tyrosine Hydroxylase purified from PC12 cells

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Ascites mouse monoclonal IgG1κ fluid containing PBS pH 7.1 , contains no preservative.
Unpurified

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation.
Anderson, GM; Beijer, P; Bang, AS; Fenwick, MA; Bunn, SJ; Grattan, DR
Endocrinology null
A FRET-based method to study protein thiol oxidation in histological preparations.
Mastroberardino, PG; Orr, AL; Hu, X; Na, HM; Greenamyre, JT
Free radical biology & medicine null
Expression patterns of the opsin 5-related genes in the developing chicken retina.
Sayuri Tomonari,Kyoichi Migita,Akira Takagi,Sumihare Noji,Hideyo Ohuchi
Developmental Dynamics null
Age and region-specific responses of microglia, but not astrocytes, suggest a role in selective vulnerability of dopamine neurons after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine exposure in monkeys.
Kanaan, NM; Kordower, JH; Collier, TJ
Glia null
Paraskevi Krashia et al.
Nature communications, 10(1), 4725-4725 (2019-10-16)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

抗体とは：抗原・エピトープ・抗体

抗体は特定の抗原と結合し、ユニークな抗原－抗体複合体を形成します。この結合の性質や、強固かつ特異的なこの結合を分子的標識として研究に使用する方法について説明します。

抗体の産生 | モノクローナル抗体とポリクローナル抗体の比較

抗体がどのように産生されるのか、クローン数、抗体のフォーマットなど、モノクローナル抗体とポリクローナル抗体の違いを説明します。

抗体基礎知識

抗体の構造、クラス、イムノグロブリンの基準値範囲に関する技術情報など、抗体を利用した作業の基礎をご説明します。

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Protocols

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

免疫組織染色（IHC）のトラブルシューティング：うまくいくコツ

発色剤を用いた明視野解析と蛍光顕微鏡法を用いたFFPEおよび凍結組織を利用した免疫組織染色（IHC）プロトコルのトラブルシューティング：うまくいくコツ

Global Trade Item Number

SKUGTIN
MAB31808436037123320

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