Anti-DLG4 Antibody

Disks large homolog 4 (UniProt P78352; also known as Postsynaptic density protein 95, PSD-95, SAP-90, SAP90, Synapse-associated protein 90) is encoded by the DLG4 (also known as PSD95) gene (Gene ID 1742) in human. PSD-95 belong to the family of membrane-associated guanylate kinases (MAGUKs), which also include PSD-93, SAP97 and SAP102. PSD95 is predominantly expressed in the hippocampus CA1 region and prefrontal cortex of the brain, where it localizes in a somatodendritic pattern in the post-synaptic membrane and in presynaptic axon terminals of inhibitory neurons. PDS-95 is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength via interactions with a diverse PSD proteins, including NMDA receptors (NMDARs), AMPA receptor (AMPAR) complexes via Stargazin/TARP, adhesion molecules, as well as other scaffolding proteins, such as GKAP and Shank. PSD95 and neuronal nitric-oxide synthase (nNOS) interaction plays a role in maintaining hypersensitivity in acute and chronic pain. In addition, the assembly of the GluR6-PSD95-CaMKII complex is implicated as a majore signaling module that mediates injury induced by brain ischemia by facilitating GluR6 serine phosphorylation by CaMKII and the activation of JNK.

~80 kDa observed. 80.50/80.47/80.47 kDa human/mouse/rat isoform 1; PSD95-alpha), 85.43/85.42 kDa (human/mouse isoform 2; PSD95-beta), 80.13/80.10 kDa (human/mouse isoform 3) calculated.

Epitope: Region between PDZ domain 2 & 3.

KLH-conjugated linear peptide corresponding to the region between PDZ domain 2 & 3 of human PSD95.

Anti-PSD95 Antibody, clone 1A8.1 is an antibody against PSD95 for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot PSD95 in human cerebral cortex and cerebellum tissue sections.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Clone 1A8.1 epitope sequence is present in all spliced isoforms of human, mouse, and rat PSD95 reported by UniProt (P78352, Q62108, P31016).

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in rat hippocampus tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSD95 in 10 µg of rat hippocampus tissue lysate.

Concentration: Please refer to lot specific datasheet.

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