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Merck

OP10

Anti-c-Myc (Ab-1) Mouse mAb (9E10)

liquid, clone 9E10, Calbiochem®

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
9E10, monoclonal
Species reactivity:
rat (weakly), human, mouse (weakly)
Application:
—
Citations:
20

Key Documents

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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

9E10, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat (weakly), human, mouse (weakly)

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Flow Cytometry (20 µg/mL)
Frozen Sections (2-10 µg/mL)
Immunoblotting (1-5 µg/mL,
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/mL,
Paraffin Sections (not recommended))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MYC(4609)
mouse ... Myc(17869)
rat ... Myc(24577)

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General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
Recognizes the ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).

Immunogen

Epitope: within amino acids 410-419
Human
a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc

Application

Flow Cytometry (20 µg/ml or use Cat. No. OP10F)

Frozen Sections (2-10 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)

Immunoprecipitation (1 µg/sample)

Chromatin Immunoprecipitation (5 µg/ml, see application references)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
HT1080 cells
Positive Control
HL-60 cells or lung carcinoma

Other Notes

LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.
The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a 45 kDa protein, but c-Myc migrates under reducing conditions as a 64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra 34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

OP10-10MG: + OP10-ML: + OP10-200UG: + OP10-MG:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kumar Sukhdeo et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(18), 7516-7521 (2007-04-25)
Multiple myeloma (MM) is an invariably fatal form of cancer characterized by clonal proliferation of malignant plasma cells in the bone marrow. The canonical Wnt signaling pathway is activated in MM cells through constitutively active beta-catenin, a messenger molecule relevant
Lixia Pu et al.
The Journal of biological chemistry, 277(49), 46877-46885 (2002-10-03)
The Cdc25 dual specificity phosphatases coordinate cell cycle progression, but potent and selective inhibitors have generally been unavailable. In the present study, we have examined one potential inhibitor, 6-chloro-7-(2-morpholin-4-ylethylamino)-quinoline-5,8-dione (NSC 663284), that was identified in the compound library of the
E Mata-Greenwood et al.
Leukemia, 16(11), 2275-2284 (2002-10-26)
Employing the natural product quassinoid brusatol, we currently report cellular and molecular events leading to cell death or terminal differentiation in a panel of leukemic cells. Brusatol and bruceantin exerted significant cytotoxic effects with several leukemic cell lines, but not
Deyu Tarika Cai et al.
Molecular membrane biology, 28(2), 90-102 (2011-01-15)
Since being introduced globally as Aspirin in 1899, acetylsalicylic acid (ASA) has been widely used as an analgesic, immune-regulatory, anti-pyretic and anti-thrombotic drug. ASA and its metabolite, salicylate, were also reported to be able to modulate antigen presenting functions of
Ling Zhu et al.
The Plant cell, 28(4), 855-874 (2016-04-14)
The phytochrome interacting factors (PIFs), a small group of basic helix-loop-helix transcription factors, repress photomorphogenesis both in the dark and light. Light signals perceived by the phytochrome family of photoreceptors induce rapid degradation of PIFs to promote photomorphogenesis. Here, we
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