Product Name
Anti-HA Affinity Matrix, from rat IgG1
biological source
rat
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
slurry
packaging
pkg of 1 mL (settled resin volume)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
capacity
2-8 nmol/mL binding capacity
storage temp.
2-8°C
Quality Level
Related Categories
Analysis Note
Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.
Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.
Application
Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
- Western blotting using the Anti-HA antibody
- Silver staining (or similar protein stain)
- In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
- Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
- Affinity column purification of HA-tagged proteins from crude protein extracts
Biochem/physiol Actions
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).
Features and Benefits
- Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
- Use nondenaturing conditions for elution of your HA-tagged protein of choice.
- Purify even rarely expressed HA-tagged proteins.
- Highly specific to YPYDVPDYA.
- Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
- Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.
General description
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Other Notes
For life science research only. Not for use in diagnostic procedures.
Physical form
1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Francisco José Taberner et al.
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David A Hubert et al.
The EMBO journal, 22(21), 5679-5689 (2003-11-01)
The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We demonstrate that specific mutations in the ATP-binding domain of a single
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The Cbl proteins (Cbl, Cbl-b, and Cbl-c) are a highly conserved family of RING finger ubiquitin ligases (E3s) that function as negative regulators of tyrosine kinases in a wide variety of signal transduction pathways. In this study, we identify a
Protocols
Anti-HA Affinity Matrix Protocol & Troubleshooting
この抗HAアフィニティーマトリックスプロトコル＆トラブルシューティングガイドでは、哺乳類、細菌および酵母細胞抽出物から得られたHAタグ付きタンパク質の免疫沈降に関する情報を提供しています。
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