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16669

Magenta-Gal

Name: Magenta-Gal
Brand: SIAL

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₅BrClNO₆

CAS Number:

93863-88-8

Molecular Weight:

408.63

PubChem Substance ID:

329751317

UNSPSC Code:

12352200

Beilstein/REAXYS Number:

1552602

MDL number:

MFCD00210022

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Properties

grade

Molecular Biology

Quality Level

100

sterility

non-sterile

assay

≥98.0% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1

InChI key

CHRVKCMQIZYLNM-RKQHYHRCSA-N

Description

General description

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

Biochem/physiol Actions

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

16669-25MG: + 16669-BULK: + 16669-100MG: + 16669-VAR:

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