Skip to Content
Merck

D1435

Dimethyl sulfoxide

meets EP testing specifications, meets USP testing specifications

Synonym(s):

DMSO

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Linear Formula:
(CH3)2SO
CAS Number:
67-68-5
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.71
PubChem Substance ID:
57654071
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
Assay:
≥99.5%
Technique(s):
cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

SMILES string

CS(C)=O

agency

meets EP testing specifications
meets USP testing specifications

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cryopreservation: suitable

impurities

≤0.1% water (Karl Fischer, USP)
≤0.2% water (Karl Fischer, EP)

color

colorless

refractive index

n20/D 1.478-1.479
n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.100-1.104 g/mL at 25 °C (relative density (EP))
1.10 g/mL (lit.)

UV absorption

λ: 285 nm Amax: ≤0.20
λ: 295 nm Amax: ≤0.20

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Dimethyl sulfoxide has been used:
  • for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments
  • as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells
  • for the suspension and separation of buccal cells
  • a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Biochem/physiol Actions

Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant. It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBP1974
SHBP1502
SHBP1501
SHBN7560
SHBL6823

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood
Chen G, et al.
Stem Cells International, 2016 (2016)
Identification of anti-viral activity of the cardenolides, Na+/K+-ATPase inhibitors, against porcine transmissible gastroenteritis virus
Yang CW, et al.
Toxicology and Applied Pharmacology, 332, 129-137 (2017)
Buccal micronucleus cytome assay: results of an intra-and inter-laboratory scoring comparison
Bolognesi C, et al.
Mutagenesis, 30(4), 545-555 (2015)
Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes
Tunccer S, et al.
Scientific reports, 8(1), 14828-14828 (2018)
Single-molecule mRNA detection and counting in mammalian tissue
Lyubimova A, et al.
Nature Protocols, 8(9), 1743-1743 (2013)
View All Related Papers

Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service