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Merck

D3435

Dey-Engley neutralizing broth

powder, suitable for microbiology, NutriSelect® Plus

Synonym(s):

D/E Neutralizing Broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
nonselective for Bacillus spp. (Spp.), nonselective for Escherichia coli, nonselective for Pseudomonas spp. (Spp.), nonselective for Salmonella Spp., nonselective for Staphylococcus Spp., nonselective for bacteria (General Media), nonselective for coliforms
Application(s):
environmental
food and beverages
pharmaceutical
sterility testing
surface monitoring, microbiology

Key Documents

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sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

final pH

7.6±0.2 (25 °C)

application(s)

environmental
food and beverages
pharmaceutical
sterility testing
surface monitoring, microbiology

storage temp.

2-8°C

suitability

nonselective for Bacillus spp. (Spp.), nonselective for Escherichia coli, nonselective for Pseudomonas spp. (Spp.), nonselective for Salmonella Spp., nonselective for Staphylococcus Spp., nonselective for bacteria (General Media), nonselective for coliforms

Quality Level

200

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Application

Dey-Engley Neutralizing Broth is used in disinfectant testing where neutralization of the antiseptics and disinfectants is important for determining bactericidal activity.
Dey-Engley neutralizing broth has been used to premoisten the sponge swab samples to neutralise the residual action of the disinfectants on the microbiological growth. Dey-Engley Neutralizing Broth is used in disinfectant testing where neutralization of the antiseptics and disinfectants for determining bactericidal activity. It has also been used to resuspend the Vermamoeba (Hartmannella) vermiformis cysts to neutralize peracetic acid (PAA).

Biochem/physiol Actions

The Dey-Engley Neutralizing Broth neutralizes a broad spectrum of antiseptics and disinfectants including, quaternary ammonium compounds, phenolics, iodine, chlorine, mercurials, formaldehyde and glutaraldehyde. Sodium thioglycollate, sodium thiosulphate, sodium bisulphite, soya lecithin and polysorbate 80 act as neutralizing components. Growth is indicated by a color change from purple to yellow.

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Dey-Engley Neutralizing Broth is used in disinfectant testing where the neutralization of the antiseptics and disinfectants is important for determining its bactericidal activity. The broth consists of tryptone that provides nitrogen and carbon sources, long chain amino acids, vitamins, and other essential nutrients. Dextrose is an energy source. Yeast extract is also a rich source of vitamin B-complex. Sodium bisulfite neutralizes aldehydes; sodium thioglycolate neutralizes mercurial; sodium thiosulfate neutralizes iodine and chlorine; lecithin neutralizes quaternary ammonium compounds; and polysorbate 80, a non-ionic surface-active agent, neutralizes substituted phenolics. Bromocresol purple is an indicator for dextrose utilization. Those organisms that ferment dextrose will turn the medium from purple to yellow. Growth of Pseudomonas sp., which does not ferment dextrose, can be detected by the formation of a pellicle on the surface of the broth.

Preparation Note

Ingredients(g/L)
Casein enzymatic hydrolysate5.00
Yeast extract2.50
Dextrose10.00
Sodium thiosulfate6.00
Sodium thioglycollate1.00
Sodium bisulfite2.50
Lecithin7.00
Polysorbate 805.00
Bromocresol purple0.02
Suspend 39 g of Dey-Engley Neutralizing Broth in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving 15 lbs. pressure (121°C) for 15 minutes.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

D3435-VAR: + D3435-BULK: + D3435-500G:4548174007778

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG8846
BCCG1677
BCCF1318
BCCD6196
BCCC6094

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Sensitivity of Vermamoeba (Hartmannella) vermiformis cysts to conventional disinfectants and protease
Fouque E, et al.
Journal of Water and Health, 13(2), 302-310 (2015)
A 10-day vacancy period after cleaning and disinfection has no effect on the bacterial load in pig nursery units
Luyckx K, et al.
BMC Veterinary Research, 12(1), 236-236 (2016)
Comparison of competitive exclusion with classical cleaning and disinfection on bacterial load in pig nursery units
Luyckx K, et al.
BMC Veterinary Research, 12(1), 189-189 (2016)
D Joseph Sexton et al.
Infection control and hospital epidemiology, 41(10), 1219-1221 (2020-07-01)
We tested 9 disinfectants against Candida auris using the quantitative disk carrier method EPA-MB-35-00: 5 products with hydrogen peroxide or alcohol-based chemistries were effective and 4 quaternary ammonium compound-based products were not. This work supported a FIFRA Section 18 emergency
H Maertens et al.
Poultry science, 98(7), 2972-2976 (2019-04-18)
Resistance to antibiotics threatens to become a worldwide health problem. An important attributing phenomenon in this context is that pathogens can acquire antibiotic resistance genes through conjugative transfer of plasmids. To prevent bacterial infections in agricultural settings, the use of
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