F5415
Ficoll® solution
Type 400, 20% in H2O
Synonym(s):
Ready-to-use density gradient media
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About This Item
sterility
0.2 μm filtered
Quality Level
type
Type 400
form
liquid
concentration
20% in H2O
technique(s)
titration: suitable
color
clear colorless
solubility
water: soluble
density
1.055-1.075 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
General description
Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.
Application
Ficoll® has been used in
- Isolation of cord blood mononuclear cells
- Isolation of CD133 negative cells
- Isolation and expansion of circulating endothelial progenitor cells.
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
The Pseudomonas aeruginosa chemotaxis methyltransferase CheR1 impacts on bacterial surface sampling.
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have