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Merck

O0625

Oil Red O

Dye content, ≥75%, certified by the Biological Stain Commission, powder

Synonym(s):

1-([4-(Xylylazo)xylyl]azo)-2-naphthol, 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol, Solvent Red 27

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About This Item

Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
1320-06-5
Molecular Weight:
408.49
NACRES:
NA.47
PubChem Substance ID:
24897931
UNSPSC Code:
12171500
EC Number:
215-295-3
MDL number:
MFCD00003898
Colour Index Number:
26125

Key Documents

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Product Name

Oil Red O, certified by the BSC

InChI key

NPGIHFRTRXVWOY-UHFFFAOYSA-N

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3

SMILES string

Cc1ccc(C)c(c1)\N=N\c2cc(C)c(cc2C)\N=N/c3c(O)ccc4ccccc34

agency

certified by the BSC

form

powder

composition

Dye content, ≥75%

technique(s)

microbe id | staining: suitable

mp

120 °C (dec.) (lit.)

solubility

chloroform/ethanol (1:1): 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Certified for use as a fat stain in Churukian′s modification of the method of Lillie and Asburn.
Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.
Oil Red O has been used:
  • for staining liver sections in histological analysis
  • for the quantification of lipids in cells
  • to stain aorta to examine atherosclerotic lesions

General description

Oil Red O is a lysochrome. It is used to stain and visualise general localization of fats.
Oil Red O stains lipid materials and the lipids take red-orange color. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, since alcohol fixation removes lipid.

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN4926
SHBN4928
SHBM5455
SHBL1039
MKCJ3586

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