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Merck

P7170

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

Synonym(s):

Ponceau S

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About This Item

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
6226-79-5
Molecular Weight:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
24898827
NACRES:
NA.47
MDL number:
MFCD00003892

Key Documents

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Product Name

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

description

for protein analysis

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

technique(s)

western blot: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Ponceau S solution has been used for membrane staining (Western blotting).

General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL7892
SLCL2343
SLCF9006
SLCB3855
SLBV4112

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Articles

タンパク質染色試薬選択ガイド

Sigmaでは、タンパク質可視化のためのEZBlue™ 試薬とProteoSilver™ 試薬をご提供しています。プロテオミクスや従来のPAGEフォーマットに適しています。

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protocols

Western Blot Protocol - Immunoblotting or Western Blot

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

ウェスタンブロッティングプロトコル － イムノブロッティングまたはウェスタンブロット

ウェスタンブロッティングプロトコルでは、研究におけるイムノブロッティング手順で極めて重要なゲルからニトロセルロースへのタンパク質転写を詳述します。

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