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03978

Methylene Blue solution

concentrate according to Ehrlich, concentrated, aqueous solution

Synonym(s):

Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
NACRES:
NA.47
PubChem Substance ID:
329747853
UNSPSC Code:
12171500
MDL number:
MFCD00012111
Beilstein/REAXYS Number:
3641570
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Quality Segment

100

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Application

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

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wgk

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flash_point_f

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flash_point_c

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ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Storage Class

12 - Non Combustible Liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

03978-250ML:4.548173388908E12

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