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18507

Atto 565 maleimide

Name: Atto 565 maleimide
Brand: SIGMA

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):

C₃₇H₃₇ClN₄O₁₀

Molecular Weight:

733.16

UNSPSC Code:

12352111

NACRES:

NA.32

MDL number:

MFCD04040675

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product line

BioReagent

Quality Level

200

assay

>90.0% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, 565 nm

fluorescence

λ_ex 563 nm; λ_em 592 nm in 0.1 M phosphate pH 7.0

λ

(ethanol with 0.1% trifluoroacetic acid)

UV absorption

λ: 560-566 nm A_max

suitability

corresponds for coupling to thiols, suitable for fluorescence

storage temp.

−20°C

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

18507-PZ: + 18507-1MG-F: + 18507-VAR-F: + 18507-1MG-BULK-F: + 18507-BULK-F:

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Journal of proteome research, 17(1), 543-558 (2017-11-14)

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Lukasz Krzemiński et al.

The journal of physical chemistry. B, 115(43), 12607-12614 (2011-09-24)

Recently, studies have been reported in which fluorescently labeled redox proteins have been studied with a combination of spectroscopy and electrochemistry. In order to understand the effect of the dye on the protein-electrode interaction, voltammetry and surface analysis have been

Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation.

Suman Lata et al.

Journal of the American Chemical Society, 128(7), 2365-2372 (2006-02-16)

Labeling of proteins with fluorescent dyes offers powerful means for monitoring protein interactions in vitro and in live cells. Only a few techniques for noncovalent fluorescence labeling with well-defined localization of the attached dye are currently available. Here, we present

Thiol reactive probes and chemosensors.

Peng H, Chen W, Cheng Y, et al.

Sensors, 12, 15907-15946 (2012)

Spectroelectrochemical investigation of intramolecular and interfacial electron-transfer rates reveals differences between nitrite reductase at rest and during turnover.

Łukasz Krzemiński et al.

Journal of the American Chemical Society, 133(38), 15085-15093 (2011-08-26)

A combined fluorescence and electrochemical method is described that is used to simultaneously monitor the type-1 copper oxidation state and the nitrite turnover rate of a nitrite reductase (NiR) from Alcaligenes faecalis S-6. The catalytic activity of NiR is measured

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