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30674

Atto 700

Name: Atto 700
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

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About This Item

UNSPSC Code:

41116100

NACRES:

NA.32

MDL number:

MFCD06798563

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product line

BioReagent

Quality Level

100

assay

≥90.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, 700 nm

fluorescence

λ_ex 681 nm; λ_em 714 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 692 nm ± 3 nm)

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 700 is a new label with high molecular absorption (1.2 x 10⁵) and quantum yield (0.25) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 700 is characterized by high photostability and thermal stability. It is also excellently soluble in water. Due to an insignificant triplet formation rate, it is well suited for single molecule detection applications.

Application

Atto 700 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, excellent thermal and photo-stability, very good water solubility and very little triplet formation. Atto 700 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.

Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

30674-BULK-F: + 30674-1MG-BULK-F: + 30674-VAR-F: + 30674-1MG-F:

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Multicolour single molecule imaging in cells with near infra-red dyes.

Tynan, C.J., et al.

PLoS ONE, 4, e36265-e36265 (2012)

Single-molecule photophysics of oxazines on DNA and its application in a FRET switch.

Jan Vogelsang et al.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 486-496 (2009-04-02)

The role and interplay of triplet states and radical ion states in single-molecule fluorescence spectroscopy has recently been elaborated providing us with new insights into the photophysics and photobleaching pathways of fluorescent dyes. Adjustment of fluorophore redox properties in combination

Identification of selective class II histone deacetylase inhibitors using a novel dual-parameter binding assay based on fluorescence anisotropy and lifetime.

Patricia Haus et al.

Journal of biomolecular screening, 16(10), 1206-1216 (2011-10-27)

Histone deacetylases (HDACs) are important epigenetic factors regulating a variety of vital cellular functions such as cell cycle progression, differentiation, cell migration, and apoptosis. Consequently, HDACs have emerged as promising targets for cancer therapy. The drugability of HDACs has been

Non-isotopic dual parameter competition assay suitable for high-throughput screening of histone deacetylases.

Daniel Riester et al.

Bioorganic & medicinal chemistry letters, 19(13), 3651-3656 (2009-05-22)

Histone deacetylases reside among the most important and novel target classes in oncology. Selective lead structures are intensively developed to improve efficacy and reduce adverse effects. The common assays used so far to identify new lead structures suffer from many

Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates.

Judith E Berlier et al.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)

Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were

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