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Merck

75784

Atto 565

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):
C31H31ClN2O9
Molecular Weight:
611.04
UNSPSC Code:
12352108
NACRES:
NA.32
MDL number:
MFCD03456130

Key Documents

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product line

BioReagent

Quality Level

100

assay

≥90.0% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 562.0-568.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 565 shows a molar extinction of 120.000 and QY of 92% in water (97% in ethanol). Decay time is 3.4 ns.
Specific and stable fluorescence labeling of histidine-tagged proteins

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

75784-BULK-F: + 75784-1MG-F: + 75784-1MG-BULK-F:

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Certificates of Analysis (COA)

Lot/Batch Number
436350/1
427722/1
439750/1

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Suman Lata et al.
Journal of the American Chemical Society, 128(7), 2365-2372 (2006-02-16)
Labeling of proteins with fluorescent dyes offers powerful means for monitoring protein interactions in vitro and in live cells. Only a few techniques for noncovalent fluorescence labeling with well-defined localization of the attached dye are currently available. Here, we present
Łukasz Krzemiński et al.
Journal of the American Chemical Society, 133(38), 15085-15093 (2011-08-26)
A combined fluorescence and electrochemical method is described that is used to simultaneously monitor the type-1 copper oxidation state and the nitrite turnover rate of a nitrite reductase (NiR) from Alcaligenes faecalis S-6. The catalytic activity of NiR is measured
Marie-Luise Humpert et al.
Proteomics, 12(12), 1938-1948 (2012-05-25)
PTMs of extracellular domains of membrane proteins can influence antibody binding and give rise to ambivalent results. Best proof of protein expression is the use of complementary methods to provide unequivocal evidence. CXCR7, a member of the atypical chemokine receptor
Takao Nakata et al.
The Journal of cell biology, 194(2), 245-255 (2011-07-20)
Polarized transport in neurons is fundamental for the formation of neuronal circuitry. A motor domain-containing truncated KIF5 (a kinesin-1) recognizes axonal microtubules, which are enriched in EB1 binding sites, and selectively accumulates at the tips of axons. However, it remains
Sebastian van de Linde et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 465-469 (2009-04-02)
We introduce a general approach for multicolor subdiffraction-resolution fluorescence imaging based on photoswitching of standard organic fluorophores. Photoswitching of ordinary fluorophores such as ATTO520, ATTO565, ATTO655, ATTO680, or ATTO700, i.e. the reversible transition from a fluorescent to a nonfluorescent state
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Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

蛍光寿命測定

蛍光寿命測定には、蛍光強度に基づく測定法にない利点があります。応用例として、蛍光寿命分析、蛍光寿命センシングおよび蛍光寿命イメージング（FLI）が挙げられます。

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