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Merck

83689

Rhodamine B

suitable for fluorescence

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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About This Item

Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
81-88-9
Molecular Weight:
479.01
EC Number:
201-383-9
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57652962
MDL number:
MFCD00011931
Colour Index Number:
45170
Beilstein/REAXYS Number:
4091619

Key Documents

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InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

form

powder or crystals

Quality Level

200

mol wt

479.01 g/mol

concentration

80-100 % (w/w) (Rhodamine B)

color

green to very dark green

mp

210-211 (dec.) (lit.)

solubility

H2O: soluble, methanol: soluble

density

0.79 g/cm3

fluorescence

λex 553 nm; λem 627 nm in methanol

suitability

in accordance for fluorescence, suitable for fluorescence

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Analysis Note

λmax. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol)

Application

Rhodamine B has been used:
  • as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
  • as a fluorescent dye in confocal microscopy
  • to study its fluorescence in water and ethanol solutions

for cross staining of N-methylamino-5-isocyanonaphthalene (MICAN)

General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.
Rhodamine B is bright-red in color.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

83689-VAR: + 83689-1G: + 83689-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF9755
BCCF1067
BCCC7998
BCBZ7334
BCBW6403

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The
A study of water circulation in Monterey harbor using rhodamine B dye (1965)
MICAN, a new fluorophore for vital and non-vital staining of human cells
Nagy Z, et al.
Toxicology in vitro, 48, 137-145 (2018)
Simplifying microfluidic separation devices towards field-detection of blood parasites
Holm SH, et al.
Analytical Methods : Advancing Methods and Applications, 8(16), 3291-3300 (2016)
The fluorescence properties of cationic rhodamine B in the gas phase
Sagoo SK and Jockusch RA
Journal of Photochemistry and Photobiology A: Chemistry, 220(2-3), 173-178 (2011)
Photocatalytic degradation of Rhodamine B dye under UV/solar light using ZnO nanopowder synthesized by solution combustion route
Nagaraja R, et al.
Powder Technology, 215, 91-97 (2012)
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