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Merck

A1908

Asialofetuin from fetal calf serum

Type II (Sigma designation)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.61
MDL number:
MFCD00130561
Form:
powder
Biological source:
bovine

Key Documents

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biological source

bovine

type

Type II (Sigma designation)

form

powder

technique(s)

activity assay: suitable, inhibition assay: suitable

impurities

salt, essentially free, ≤1.0% N-acetylneuraminic acid

solubility

H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow

storage temp.

2-8°C

Quality Level

200

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Application

Asialofetuin from fetal calf serum is suitable for use:
  • in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
  • in the inhibition of zona pellucida hardening of mouse oocytes
  • as an acceptor in sialyltransferase enzyme activity assay
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Biochem/physiol Actions

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.
Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.

Preparation Note

Enzymatic cleavage using neuraminidase

flash_point_f

Not applicable

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

A1908-50MG: + A1908-10MG: + A1908-5MG: + A1908-VAR: + A1908-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK5437
SLBD4547V
027K7418
093H9510
079F9640

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In vitro gene delivery mediated by asialofetuin-appended cationic liposomes associated with gamma-cyclodextrin into hepatocytes
Motoyama K, et al.
Journal of Drug Delivery, 2011 (2011)
X C Wang et al.
The Journal of biological chemistry, 264(3), 1854-1859 (1989-01-25)
The hepatic acute phase response is accompanied by increased levels of Gal beta 1-4GlcNAc alpha 2,6-sialyltransferase activity in liver and in circulation. Previous studies suggested that cytokines and glucocorticoids mediate the induction of this sialyltransferase activity. In this study the
Serum-resistant lipoplexes in the presence of asialofetuin
Liposomes, 2011, 425-434 (2010)
W M Brown et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(11), 749-755 (1992-11-01)
Bovine fetuin, the first fetal protein to be described, has recently been shown to be a species homologue of a well known human plasma protein--alpha 2HS glycoprotein (alpha 2HS). The fetuins are now known to be members of the cystatin
Xinhua Lin et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 24(11), 2051-2058 (2006-08-22)
These studies evaluated whether F2A4-K-NS, a peptide mimetic of FGF-2, could augment ectopic bone production following the subcutaneous implant of human demineralized bone matrix (DBM). DBM was formulated into a gel with and without F2A4-K-NS, and injected subcutaneously into athymic
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