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Merck

A7921

Agar

suitable for plant cell culture, powder

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
UNSPSC Code:
50405901
eCl@ss:
42040102
EC Number:
232-658-1
NACRES:
NA.21
MDL number:
MFCD00081288

Key Documents

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SMILES string

O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

biological source

algae (Rhodophyceae)

form

powder

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

storage temp.

room temp

Quality Level

200

application(s)

agriculture
food and beverages
life science and biopharma

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Application

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

A7921-2.5KG: + A7921-100G: + A7921-500G: + A7921-1KG: + A7921-VAR: + A7921-BULK:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG2344
BCCF0092
BCCD6438
BCCC6051
BCCB9132

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Plant Tissue Culture, Development, and Biotechnology, 21(11), 1577-1577 (2011)
AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus
Rabaiolli SMS, et al.
CERNE, 23(2), 185-192 (2017)
Thidiazuron induced micropropagation of Hypericum triquetrifolium Turra
Oluk, E and Orhan, Serpil
African Journal of Biotechnology, 8(15), 771-777 (2009)
A new transformation-regeneration procedure in the model legume Lotus japonicus: root explants as a source of large numbers of cells susceptible to Agrobacterium-mediated transformation
Lombari P, et al.
Plant Cell Reports, 21(8), 771-777 (2003)
Bety Shiue de Hsie et al.
Journal of photochemistry and photobiology. B, Biology, 198, 111577-111577 (2019-08-04)
Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was
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