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Merck

A9205

Bovine Serum Albumin solution

30% in saline, fatty acid free, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
NACRES:
NA.27
MDL number:
MFCD00130384

Key Documents

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Product Name

Bovine Serum Albumin solution, 30% in saline, fatty acid free, aseptically filled

storage temp.

2-8°C

Quality Level

200

biological source

bovine

sterility

aseptically filled

assay

28-32% protein basis (biuret)

form

solution

purified by

cold ethanol fractionation

does not contain

stabilizer or preservative

origin

USA origin

concentration

28-32% protein (biuret)
30% in saline

free fatty acid content

≤0.01%

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

impurities

≤0.01% free fatty acid

UniProt accession no.

P02769

foreign activity

BT virus, none detected
VSV virus, none detected

Gene Information

bovine ... ALB(280717)

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Application

Bovine Serum Albumin solution has been used:
  • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
  • as a component of hepatocyte recovery medium
  • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

General description

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Preparation Note

Initial fractionation by cold alcohol precipitation

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ3171
SLCH0318
SLCC4115
SLCC1678
SLBV4868

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Effects of different states of sheep fetal fibroblasts as donor cells on the early development in vitro of reconstructed sheep embryos
Wang H, et al.
Science in China. Series C, Life Sciences / Chinese Academy of Sciences, 50(2), 178-185 (2007)
Pilar Argente-Arizón et al.
Endocrinology, 159(1), 368-387 (2017-10-28)
Astrocytes participate in both physiological and pathophysiological responses to metabolic and nutrient signals. Although most studies have focused on the astrocytic response to weight gain due to high-fat/high-carbohydrate intake, surplus intake of a balanced diet also induces excess weight gain.
Yi Fan et al.
SLAS discovery : advancing life sciences R & D, 23(7), 742-750 (2018-06-07)
Enhancing antitumor activities of the human immune system is a clinically proven approach with the advent of monoclonal antibodies recognizing programmed cell death protein-1 (PD1) receptors on immune cell surfaces. Historically, using flow cytometry as a means to assess next-generation
Jing-Syuna Ruan et al.
The Journal of nutritional biochemistry, 59, 37-48 (2018-07-01)
Lipotoxicity is associated with a high level of fatty acid accumulation in pancreatic β-cells. An overload of free fatty acids contributes to pancreatic β-cell apoptosis and dysfunction. Insulin secretion involves sequential ionic events upon glucose stimulation. ATP sensitive potassium (KATP)
Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1
Nadal A, et al.
The Biochemical Journal, 366(1), 289-297 (2002)
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