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B7435

CelLytic™ B Cell Lysis Reagent

For bacterial cell lysis, standard strength

Synonym(s):

Cell lysis reagent

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.77
MDL number:
MFCD02097909
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Quality Segment

200

form

solution

input

bacteria and yeast sample(s)

Application

A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
CelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.

Features and Benefits

  • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
  • Scalable for 1 to 25 grams of bacterial cell paste
  • No interference with downstream applications such as affinity chromatography, IP, and Western blotting
  • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Other Notes

Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

B7435-500ML: + B7435-BULK: + B7435-500ML-KC: + B7435-50ML-KC: + B7435-50ML: + B7435-500ML-EW: + B7435PROC: + B7435-VAR:

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