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Merck

B8438

Alcian Blue solution

1% in 3% acetic acid, pH 2.5

Synonym(s):

Alcian Blue 8XG solution

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About This Item

NACRES:
NA.47
UNSPSC Code:
12171500

Key Documents

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Product Name

Alcian Blue solution, 1% in 3% acetic acid, pH 2.5

form

liquid

concentration

1% in 3% acetic acid, pH 2.5

technique(s)

microbe id | staining: suitable

color

dark blue to very dark blue

pH range

2.4-2.6

Amax

0.45-0.60 (1:400 in H2O)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

Related Categories

Application

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Biochem/physiol Actions

Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

B8438-500ML:4548173954554 + B8438-250ML:4548173954547 + B8438-BULK: + B8438-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCQ8200
MKCQ4903
MKCQ4904
MKCQ0449
MKCP7401

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A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
The Biochemical Journal, 131(2), 351-357 (1973)
Mario Barilani et al.
Cell transplantation, 25(8), 1501-1514 (2016-02-07)
During the last decade it has been demonstrated that mesenchymal progenitors are present and can be isolated also from cord blood (CB). Recently, we managed to set up a standard protocol allowing the isolation of mesenchymal stromal cells (MSCs) with
Hao Wu et al.
Experimental cell research, 361(2), 324-332 (2017-11-04)
Mesenchymal stem-cell based therapies have been proposed as novel treatments for intervertebral disc (IVD) degeneration. The development of these treatment strategies, however, has been hindered by the incomplete understanding of the origin, biological properties of nucleus pulposus (NP) derived stem/progenitor
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