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Merck

B9146

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%, powder, suitable for identification of lac+ bacterial colonies

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
NACRES:
NA.83
PubChem Substance ID:
57653968
UNSPSC Code:
12352204
EC Number:
230-640-8
MDL number:
MFCD00005666
Beilstein/REAXYS Number:
1402009

Key Documents

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Product Name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

assay

≥98%

form

powder

solubility

DMF: methanol (1:1): soluble 50 mg/mL

suitability

suitable for identification of lac+ bacterial colonies

storage temp.

−20°C

Quality Level

200

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Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Preparation Note

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL8552
SLCK8733
SLCJ4711
SLCH7106
SLCD7060

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Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

酵母形質転換のプロトコル

酵母は成長が速く、細胞が分散しているため、真核生物の研究に適したモデル生物と考えられています。

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