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Merck

B9643

Bicinchoninic Acid solution

Synonym(s):

BCA

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352106
Form:
liquid

Key Documents

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form

liquid

suitability

suitable for determination of protein concentration

Quality Level

200

General description

Protein determination is one of the most common operations performed in biochemical research. Bicinchoninic acid solution is used in the bicinchoninic acid (BCA) assay, which is equivalent to the Lowry procedure. Both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. The BCA assay is more sensitive and applicable than either biuret or Lowry procedures.

Application

Bicinchoninic Acid solution (Bicinchoninic acid protein assay kit) is used :
  • For the determination of total protein content in the process of quantifying bromelain, extracted from the pineapple stem residues using aqueous micellar two-phase systems composed of ionic liquids as co-surfactants.
  • For the determination of bovine serum albumin (BSA) in biomimetic calcium phosphate coating co-precipitated on titanium alloy plates.

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

Features and Benefits

  • It is easy to use.
  • The color complex is stable.
  • There is less susceptibility to detergents.
  • It is applicable over a broad range of protein concentrations.

Packaging

Also available as part of the Bicinchoninic Acid Kit for Protein Determination (BCA-1).

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

B9643-1L-KC: + B9643-VAR: + B9643-BULK: + B9643-1L:

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Certificates of Analysis (COA)

Lot/Batch Number
SHBD1781V
SLCK3724
SLCG8086
SLCD5350
SLCB6552

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Diego García-Ayuso et al.
Experimental eye research, 161, 10-16 (2017-05-30)
In this work we study the effects of an acute light-induced retinal degeneration on the population of melanopsin positive retinal ganglion cells (m
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente AF, et al.
Process Biochemistry (Oxford, United Kingdom), 51(4), 528-534 (2016)
Shabnam Khatibi et al.
Growth factors (Chur, Switzerland), 35(2-3), 100-124 (2017-09-28)
Mathematical models for TGF-β and IL-6 signalling have been linked, providing a platform for analyzing the crosstalk between the systems. An integrated IL-6:TGF-β model was developed via a reduced set of reaction equations which incorporate both feedback loops and appropriate
Shabnam Khatibi et al.
BMC systems biology, 11(1), 48-48 (2017-04-15)
Transforming growth factor β (TGF-β) signalling regulates the development of embryos and tissue homeostasis in adults. In conjunction with other oncogenic changes, long-term perturbation of TGF-β signalling is associated with cancer metastasis. Although TGF-β signalling can be complex, many of
Zhenhua Ma et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 216, 29-37 (2018-11-12)
This study explored the impact of fishmeal replacement by commercial soybean meal (SM) and EnzoMeal (EZM) on Asian seabass Lates calcarifer growth performance using six diets. The six diets comprised two sources of plant proteins with three levels each, including
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Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Related Content

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

タンパク質定量

さまざまなサンプルのタンパク質濃度を正確に測定するためのタンパク質定量法、試薬、イムノアッセイ技術。

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Product Information Sheet - B9643

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