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Merck

B9673

1-Bromo-3-chloropropane

for isolation of RNA

Synonym(s):

1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide

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About This Item

Linear Formula:
Cl(CH2)3Br
CAS Number:
109-70-6
Molecular Weight:
157.44
UNSPSC Code:
41105515
NACRES:
NA.52
PubChem Substance ID:
24892149
EC Number:
203-697-1
Beilstein/REAXYS Number:
605278
MDL number:
MFCD00000998
Grade:
Molecular Biology
Assay:
≥98.5% (GC)
Bp:
144-145 °C (lit.)

Key Documents

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Product Name

1-Bromo-3-chloropropane, for isolation of RNA

SMILES string

ClCCCBr

InChI key

MFESCIUQSIBMSM-UHFFFAOYSA-N

InChI

1S/C3H6BrCl/c4-2-1-3-5/h1-3H2

grade

Molecular Biology

assay

≥98.5% (GC)

form

liquid

refractive index

n20/D 1.486 (lit.)

bp

144-145 °C (lit.)

density

1.592 g/mL at 25 °C (lit.)

storage temp.

room temp

Quality Level

200

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Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
  • feline teeth
  • mice brain and liver tissues
  • human ectocervical epithelial cells
  • placental samples
  • human mesenchymal stromal cells (hMSCs)

Features and Benefits

  • less toxic alternative to chloroform
  • compatible with TRI reagent for isolation of RNA with decreased DNA contamination

General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
MKCM4419
MKCL6404
MKCH2273
MKCF4305
MKCD0909

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Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
Lashkari BS and Anumba DOC
PLoS ONE, 12(3), e0173646-e0173646 (2017)
Ghalia Boubaker et al.
PLoS neglected tropical diseases, 14(5), e0007640-e0007640 (2020-05-23)
We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection of experimental primary alveolar echinococcosis (AE), a parasitic infection caused upon ingestion of E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice
Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation.
P Chomczynski et al.
Analytical biochemistry, 225(1), 163-164 (1995-02-10)
Isolation of total RNA from transgenic mouse melanoma subsets using fluorescence-activated cell sorting
Microarray methods for drug discovery, 27-44 (2010)
Neuroprotection Induced by Energy and Protein-Energy Undernutrition Is Phase-Dependent After Focal Cerebral Ischemia in Mice
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Protocols

TRI Reagent® Protocol

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent®のプロトコル

TRI Reagent®では、サンプル調製ガイドラインとトラブルシューティングを用いてDNA、RNA、タンパク質の同時分離が実現します。

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