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Merck

BCK-EDU594

ClickTech EdU Cell Proliferation Kit 594 for IM

sufficient for 100 assays

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NACRES:
NA.22
UNSPSC Code:
12161503

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Product Name

ClickTech EdU Cell Proliferation Kit 594 for IM, sufficient for 100 assays

fluorescence

λex 584 nm; λem 603 nm

shipped in

wet ice

storage temp.

2-8°C

Application

Imaging

General description

EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the Baseclick EdU-Click Assays utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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prtr

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fsl

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ishl_indicated

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ishl_notified

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Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

Articles

Cell Proliferation EdU Assay for DNA Synthesis Detection

Explore the EdU Cell Proliferation Assay for fast DNA synthesis detection in cell biology, oncology, and drug research.

Related Content

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.

Product Information Sheet - BCKEDU594

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