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Merck

C0543

Mouse Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

Synonym(s):

Type IV collagen, EHS-collagen

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About This Item

CAS Number:
9007-34-5
UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
MDL number:
MFCD00130825
Biological source:
mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable

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Product Name

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

type

Type IV (Miller)

product line

BioReagent

form

lyophilized powder

packaging

glass bottle of 1 vial

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt accession no.

B1AVK5, P02463, P08122, Q63ZW6, Q9QZS0

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Related Categories

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Application

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
  • in adhesion assays.
  • in hydrogel fabrication.
  • to coat cell culture plates for cell differentiation.
  • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.
Recommended for use as a cell culture substratum at 6-10 μg/cm2.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Preparation Note

0.75 mg/vial
Chloroform treated.
Pepsin is not used in the preparation.

Other Notes

Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C0543-VAR: + C0543-1VL: + C0543-BULK: + C0543-1VL-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
038M4014V
11170102
106M4061V
11160521
035M4031V

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Evaluation of cell binding activities of Leptospira ECM adhesins.
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PLoS Neglected Tropical Diseases, 9(4), e0003712-e0003712 (2015)
Gels Handbook: Fundamentals, Properties, Applications null
Mimicking the Extracellular Matrix: The Intersection of Matrix Biology and Biomaterials (2015)
Cell Motility in Cancer Invasion and Metastasis (2006)
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to
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