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Merck

C0858

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

Synonym(s):

COX-2, Prostaglandin H synthase 2

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
MFCD00071378
Specific activity:
≥8000 units/mg protein
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells., expressed in insect cells

Key Documents

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recombinant

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells., expressed in insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

specific activity

≥8000 units/mg protein

mol wt

dimer subunit mol wt 70-74 kDa

UniProt accession no.

P35354

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... PTGS2(5743)

General description

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Physical form

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol

Other Notes

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.
Tagged with a six-residue histidine sequence near the N-terminus.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act Listed

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C0858-1000UN-PW: + C0858-1000UN-LBL: + C0858-1000UN: + C0858-BULK: + C0858-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK4738
SLBZ1423
SLBV9863
SLBV1459
SLBP5262V

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María J Pérez et al.
Journal of food science, 83(5), 1454-1462 (2018-04-17)
This work is part of the search in native food matrices from arid regions of Argentina of interest to improve human health. Prosopis species are ethnic food resources in South America capable of growing in arid and semi-arid environments. This
Masanori Sugimoto et al.
Drug development research, 77(1), 20-28 (2016-01-15)
Preclinical Research This article describes the properties of a novel topical NSAID (Nonsteroidal anti-inflammatory drug) patch, SFPP (S(+)-flurbiprofen plaster), containing the potent cyclooxygenase (COX) inhibitor, S(+)-flurbiprofen (SFP). The present studies were conducted to confirm human COX inhibition and absorption of
Alejandra Moreno et al.
Natural product communications, 10(6), 991-994 (2015-07-23)
Zuccagnia punctata Cav. (Fabaceae) is an Argentine medicinal aromatic shrub (jarilla pispito, puspus, lata and jarilla macho). The chalcones were identified as pigments responsible for the yellow color of the flowers. Hydroethanolic extracts were obtained both from fresh flowers and
Kaneez Fatima et al.
Frontiers in nutrition, 9, 857256-857256 (2022-04-26)
Carvacrol, a monoterpene known for its pharmacological activities, is present in the essential oil of Origanum majorana, Origanum vulgare, Thymus vulgaris, and Lippia graveolens. It is used in food as a flavoring and preservative agent in cosmetics and medicines because
Tetsuo Kida et al.
PloS one, 9(5), e96481-e96481 (2014-05-07)
To evaluate the pharmacokinetics and efficacy of topically applied nonsteroidal anti-inflammatory drugs (NSAIDs) in the retinochoroidal tissues of rabbits. The cyclooxygenase (COX) inhibitory activity of diclofenac, bromfenac, and amfenac, an active metabolite of nepafenac, were determined using human-derived COX-1 and
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