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Merck

C1655

Cholera Toxin B subunit

GM1-binding, lyophilized powder

Synonym(s):

CTB

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About This Item

UNSPSC Code:
12352200
PubChem Substance ID:
329774902
NACRES:
NA.77
MDL number:
MFCD02356522
Form:
lyophilized powder
Quality level:
200

Key Documents

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Product Name

Cholera Toxin B subunit, FITC conjugate, lyophilized powder

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

conjugate

FITC conjugate

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~20% Lowry

extent of labeling

~1.0 mol FITC per mol protein

storage temp.

2-8°C

Quality Level

200

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Analysis Note

Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
  • to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat
  • to stain epididymal sperms of mice
  • to stain peritoneal macrophages of mice

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H332,H412

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Poisonous substance

pdsc

Class I Designated Chemical Substances

prtr

C1655-250UG-PW: + C1655-250UG:4548173343921 + C1655-VAR: + C1655-500UG: + C1655-.5MG:4548173343914 + C1655-.5MG-PW: + C1655-BULK:

jan

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Certificates of Analysis (COA)

Lot/Batch Number
109M4073V
049M4142V
109M4072V
11190214
088M4101V

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