C2687
Monoclonal Anti-Calponin antibody produced in mouse
clone hCP, ascites fluid
Synonym(s):
Anti-Calponin-1
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About This Item
Technique(s):
western blot: 1:10,000 using human uterus extract, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable
Conjugate:
unconjugated
Application:
ARR, IHC (p), IP, WB
Clone:
hCP, monoclonal
Citations:
91
Species reactivity:
rat, rabbit, pig, human, mouse
Uniprot accession no.:
technique(s)
western blot: 1:10,000 using human uterus extract, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable
isotype
IgG1
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
hCP, monoclonal
mol wt
antigen 34 kDa
contains
15 mM sodium azide
species reactivity
rat, rabbit, pig, human, mouse
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CNN1(1264), CNN2(1265)
mouse ... Cnn1(12797), Cnn2(12798)
rat ... Cnn1(65204), Kcnip4(259243)
Application
Monoclonal Anti-Calponin antibody produced in mouse is suitable for the following applications:
- Immunohistochemistry using formalin-fixed, paraffin-embedded sections
- Immunoprecipitation
- Microarray
- Western blotting (at a dilution of 1:10,000 using human uterus extract)
- Flow cytometry analysis
Biochem/physiol Actions
Calponin is a calcium binding protein that belongs to a family of actin-associated proteins. Calponin is necessary for autophosphorylation of protein kinase C (PKC) in vascular smooth muscle (VSM). Calponin-1 inhibition can prevent uterine smooth muscle cell migration, cause morphological change and rearrange F-actin without affecting its proliferation and apoptosis. Calponin h1 (CN) is a differentiation marker of smooth muscle cells and is down-regulated in the blood vessels of several human tumors. It can inhibit actomyosin ATPase activity. The h1 and h2 calponins bind F-actin and play a key role in regulating actin filaments in smooth muscle and non-muscle cells.
The antibody hCP (also cited as CALP), localizes calponin in mammalian smooth muscle. In human uterus, an additional band of approximately 27 kDa (l-calponin) may also be stained. The antibody does not cross-react with skeletal, cardiac or non-muscle tissue calponin. Nevertheless, the antibody exhibits cross-reactivity with an epitope (150 kDa range) in human or mouse skeletal muscle. In immunohistochemical staining, the product exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells in tissue sections and primary cultured cells (or early passages), but not most cell lines originally derived from smooth muscle. The antibody does not stain epithelial, endothelial or connective tissue fibroblast cells. This product does not cross react with smooth muscle tissue from chicken.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
human uterus smooth muscle extract.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
C2687-.2ML: + C2687-100UL: + C2687-VAR: + C2687-BULK: + C2687-.5ML:
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Yong-hong Gu et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 30(6), 1369-1372 (2010-06-30)
To study the effects of calponin-1 expression inhibition on the proliferation , invasiveness, apoptosis and cytoskeleton of uterine smooth muscle cells, and explore the molecular mechanism of calponin-1 in the uterine smooth muscle cells for labor onset. siRNA-calponin-1 adenovirus plasmid
Guoqing Hu et al.
The Journal of biological chemistry, 285(16), 11800-11809 (2010-02-20)
Fully differentiated mature smooth muscle cells (SMCs) are characterized by the presence of a unique repertoire of smooth muscle-specific proteins. Although previous studies have shown myocardin to be a critical transcription factor for stimulating expression of smooth muscle-specific genes, the
Jian-Ping Jin et al.
Protein expression and purification, 31(2), 231-239 (2003-10-11)
Three homologous calponin isoforms, named h1, h2, and acidic calponins, have been found in birds and mammals. Based primarily on studies of chicken gizzard smooth muscle (h1) calponin, calponin has been identified as a family of actin-associated proteins that inhibit
Xiaoke Yin et al.
Proteomics, 6(24), 6437-6446 (2006-12-14)
Embryonic stem (ES) cells can differentiate into vascular smooth muscle cells (SMCs), but differences in protein composition, function and behaviour between stem cell-derived and mature SMCs remain to be characterized. Using differential in gel electrophoresis (DIGE) and MS, we identified
Y Yanagisawa et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 34(5), 531-537 (2007-08-21)
Calponin h1 (CN) is a differentiation marker of smooth muscle cells that has been reported to be down-regulated in the blood vessels of several human tumors. In this study, we examined CN expression in blood vessels in relation to the
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