C2867
Cytochrome c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture
Synonym(s):
CYC, CytC, Cytochrome c from horse heart
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About This Item
Form:
powder
Assay:
≥99% (SDS-PAGE)
Biological source:
horse heart
Mol wt:
12,384
biological source
horse heart
Quality Level
product line
BioUltra
assay
≥99% (SDS-PAGE)
form
powder
mol wt
12,384
storage condition
(Tightly closed Dry)
technique(s)
cell culture | mammalian: suitable
solubility
water: 10 mg/mL, dark red-brown
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
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General description
Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria.
Research area: Apoptosis
Research area: Apoptosis
Application
Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.
Biochem/physiol Actions
Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C.
Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen
Preparation Note
A further purification of C2506
Prepared using TCA.
Other Notes
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
C2867-10MG: + C2867-VAR: + C2867-BULK: + C2867-50MG:
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Yuning Hong et al.
Journal of the American Chemical Society, 134(45), 18713-18723 (2012-10-17)
Interactions of cytochrome c (cyt c) with cardiolipin (CL) partially unfold the protein, activating its peroxidase function, a critical event in the execution of apoptosis. However, structural features of the altered protein species in the heterogeneous ensemble are difficult to
Raja Ghosh et al.
Journal of chromatography. A, 1515, 138-145 (2017-08-13)
In a recent paper, box-shaped or cuboid packed bed devices have been proposed as alternative to columns for chromatographic separations. We first propose a mathematical model for residence time distribution in such devices. Based on it, we examine factors likely
Luz P Blanco et al.
Arthritis & rheumatology (Hoboken, N.J.), 73(12), 2282-2292 (2021-05-14)
Neutrophil extracellular traps (NETs) are extracellular lattices composed of nucleic material bound to neutrophil granule proteins. NETs may play pathogenic roles in the development and severity of autoimmune diseases such as systemic lupus erythematosus (SLE), at least in part, through
Diana Bahhir et al.
PLoS genetics, 15(10), e1008410-e1008410 (2019-10-05)
Mitochondria have been increasingly recognized as a central regulatory nexus for multiple metabolic pathways, in addition to ATP production via oxidative phosphorylation (OXPHOS). Here we show that inducing mitochondrial DNA (mtDNA) stress in Drosophila using a mitochondrially-targeted Type I restriction
Reiko Ban-Ishihara et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(29), 11863-11868 (2013-07-04)
Mammalian cells typically contain thousands of copies of mitochondrial DNA assembled into hundreds of nucleoids. Here we analyzed the dynamic features of nucleoids in terms of mitochondrial membrane dynamics involving balanced fusion and fission. In mitochondrial fission GTPase dynamin-related protein
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