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Merck

C3228

CelLytic™ MT Cell Lysis Reagent

For mammalian tissues

Synonym(s):

Cell lysis buffer, Cell lysis reagent

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About This Item

NACRES:
NA.56
UNSPSC Code:
41116134

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Application

CelLytic™ MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Features and Benefits

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Analysis Note

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Other Notes

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C3228-500ML: + C3228-VAR: + C3228-50ML: + C3228-500ML-PW: + C3228-BULK: + C3228-50ML-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
099M4092V
11190414
059M4132V
059M4131V
11181119

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M Espe et al.
SpringerPlus, 4, 658-658 (2015-11-07)
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Sebori R, et al.
Oxidative Medicine and Cellular Longevity, 2018 (2018)
Leticia E Camacho et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(2), R101-R109 (2017-05-12)
Intrauterine growth restriction (IUGR) is associated with persistent metabolic complications, but information is limited for IUGR infants. We determined glucose-stimulated insulin secretion (GSIS) and insulin sensitivity in young lambs with placental insufficiency-induced IUGR. Lambs with hyperthermia-induced IUGR (
Quantitative expression and localization of GABAB receptor protein subunits in hippocampi from patients with refractory temporal lobe epilepsy
Sheilabi MA, et al.
Neuropharmacology, 136, 117128-117128 (2018)
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The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a
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Product Information Sheet - C3228

Instructions

Protein Research Guide

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CelLytic Protein Extraction Reagents
Product Information Sheet - C3228

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