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Merck

C3867

Rat Collagen Type I

from rat tail, liquid, 4 mg/mL, suitable for cell culture

Synonym(s):

Collagen from rat tail, Col1a1

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About This Item

CAS Number:
9007-34-5
UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
MDL number:
MFCD00130825
Biological source:
rat tail
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
>0.5 mg/mL Biuret
Mol wt:
apparent mol wt 115-130 kDa by SDS-PAGE (doublet), apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
Assay:
>95% (SDS-PAGE)

Key Documents

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Product Name

Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered

biological source

rat tail

description

Approx. 100mg/vial ((35 - 40 ml) )

sterility

sterile-filtered

product line

BioReagent

assay

>95% (SDS-PAGE)

form

liquid

mol wt

apparent mol wt 115-130 kDa by SDS-PAGE (doublet), apparent mol wt 215-235 kDa by SDS-PAGE (doublet)

packaging

pkg of 1 vial

concentration

>0.5 mg/mL Biuret

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

impurities

Mycoplasma Test by Barile, none detected

solubility

soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)

NCBI accession no.

NM_053304.1

UniProt accession no.

P02454

binding specificity

Peptide Source: Collagen
, Peptide Source: Fibronectin

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Gene Information

rat ... Col1a1(29393)

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General description

Collagen is an extracellular matrix protein comprising triple helical structure. The structure of collagen type I, in particular, is characterized by the presence of three identical α-chains in its homotrimetric form. Among these three α-chains, one exhibits slight sequence variation. Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain.

Application

Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line and podocytes. It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel.
Used as a coating material to support adherent cells growth and differentiation.

Biochem/physiol Actions

Collagen protein is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes.

Features and Benefits

  • Sourced from rat tail.
  • Sterile-filtered to minimize contamination risks.
  • Suitable for a wide range of cell culture applications.
  • Provides optimal surface coverage (6-10 μg/cm2) for promoting cell adhesion and proliferation.

Preparation Note

Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.
Sterile solution prepared from rat tail tendons by a modification of the published extraction method 1. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method 2.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
When C3867 is first removed from the cooler it will appear gelled. When it is allowed to come fully to 37°C it will appear as a liquid.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

C3867-1VL: + C3867-VAR: + C3867PROC: + C3867-1VL-PW: + C3867-BULK: + C3867-PH:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK5464
SLCJ8114
SLCH6328
SLCG2117
SLCF0971

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Mechanisms of angiotensin II signaling on cytoskeleton of podocytes
Hsu HH, et al.
Journal of Molecular Medicine, 86(12), 1379-1394 (2008)
Ectopic adenine nucleotide translocase activity controls extracellular ADP levels and regulates the F1-ATPase-mediated HDL endocytosis pathway on hepatocytes
Cardouat G, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(9), 832-841 (2017)
On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids
Mattei G, et al.
Testing, 12(3), e0173206-e0173206 (2017)
Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications
Rajan N, et al.
Nature Protocols, 1(6), 2753-2753 (2006)
Thermal helix-coil transition in UV irradiated collagen from rat tail tendon
Sionkowska A and Kaminska A
International Journal of Biological Macromolecules, 24(4), 337-340 (1999)
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Articles

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D細胞培養のための接着因子

ここでは、細胞外基質（ECM）とその接着因子構成成分の構造生物学的な機能のほか、in vitroでの応用向けにどのようなものが利用できるかについて考察します。

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