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C6164

Cell Freezing Medium-DMSO 1×

Name: Cell Freezing Medium-DMSO 1×
Brand: SIGMA

sterile-filtered, suitable for cell culture

Synonym(s):

cryopreservation medium

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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sterility

sterile-filtered

Quality Level

100

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

Related Categories

Cell Freezing Reagents

Classical Media & Buffers

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Culture Supplements & Reagents

General description

Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).

Application

Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

C6164-50ML-PW: + C6164-BULK: + C6164-6X50ML: + C6164-VAR: + C6164-50ML: + C6164-50ML-LBL:

jan

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Safety and immunogenicity of oral microencapsulated enterotoxigenic Escherichia coli surface antigen 6 (CS6) with and without LTR192G: a randomized clinical trial

Lapa JA, et al.

Clinical and Vaccine Immunology : CVI (2008)

Variations of components of the plasminogen activation system with the cell cycle in benign prostate tissue and prostate cancer

Plas E, et al.

Cytometry, 46(3), 184-189 (2001)

Invasion of human breast cancer cells in vivo requires both paracrine and autocrine loops involving the colony-stimulating factor-1 receptor.

Antonia Patsialou et al.

Cancer research, 69(24), 9498-9506 (2009-11-26)

Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased

Directional freezing for the cryopreservation of adherent mammalian cells on a substrate

Bahari L, et al.

Testing, 13(2), e0192265-e0192265 (2018)

PolyQ repeat expansions in ATXN2 associated with ALS are CAA interrupted repeats.

Zhenming Yu et al.

PloS one, 6(3), e17951-e17951 (2011-04-12)

Amyotrophic lateral sclerosis (ALS) is a devastating, rapidly progressive disease leading to paralysis and death. Recently, intermediate length polyglutamine (polyQ) repeats of 27-33 in ATAXIN-2 (ATXN2), encoding the ATXN2 protein, were found to increase risk for ALS. In ATXN2, polyQ

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Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Protocols

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

細胞株の凍結保存

DMSOなどの凍結保存剤を用いて高い生存率で凍結するための細胞培養プロトコル。ハンドブックの無料ダウンロード。