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Merck

C6198

Anti-Actin, α-Smooth Muscle - Cy3™ antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Actin, α-Smooth Muscle, Alpha-actin 2 Cy3, alpha-smooth muscle actin - Cy3™ antibody, SMA

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Actin, α-Smooth Muscle - Cy3™ antibody, Mouse monoclonal, clone 1A4, purified from hybridoma cell culture

biological source

mouse

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, mouse, rat, chicken, frog, canine, guinea pig, rabbit, bovine, goat, sheep, snake

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix

isotype

IgG2a

UniProt accession no.

P62736

application(s)

research pathology

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Biochem/physiol Actions

Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
059M4797V
22190105
058M4761V
127M4859V
22170945

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Coexpression of alpha-sarcomeric actin, alpha-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
Babai F, et al.
Differentiation, 44(2), 132-142 (1990)
Jinhua Li et al.
The American journal of pathology, 175(4), 1380-1388 (2009-09-05)
Diabetic nephropathy is the leading cause of chronic renal failure. Myofibroblasts play a major role in the synthesis and secretion of extracellular matrix in diabetic renal fibrosis. Increasing evidence suggests that endothelial cells may undergo endothelial-myofibroblast transition under physiological and
C Konoeda et al.
Transplantation proceedings, 45(5), 1797-1801 (2013-06-19)
Aberrant epithelial repair is a crucial event in the airway remodeling that characterizes obliterative bronchiolitis (OB) in transplanted lungs. Recent data from experiments using epithelial cell lines and human airway tissues from lung transplant recipients suggest that epithelial to mesenchymal
Debby Gawlitta et al.
Tissue engineering. Part A, 18(1-2), 208-218 (2011-08-24)
To ensure the survival of engineered bone after implantation, we combined human endothelial colony forming cells (ECFCs) and multipotent stromal cells (MSCs) as a proof of concept in a co-culture model to create in vitro prevascularized bone constructs. We hypothesized
Patricia Parsons-Wingerter et al.
The American journal of pathology, 167(1), 193-211 (2005-06-24)
Integrin alpha5beta1 is among the proteins overexpressed on tumor vessels and is a potential target for diagnostics and therapeutics. Here, we mapped the distribution of alpha5beta1 integrin in three murine tumor models and identified sites of expression that are rapidly
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抗体とは：抗原・エピトープ・抗体

抗体は特定の抗原と結合し、ユニークな抗原－抗体複合体を形成します。この結合の性質や、強固かつ特異的なこの結合を分子的標識として研究に使用する方法について説明します。

抗体の産生 | モノクローナル抗体とポリクローナル抗体の比較

抗体がどのように産生されるのか、クローン数、抗体のフォーマットなど、モノクローナル抗体とポリクローナル抗体の違いを説明します。

抗体基礎知識

抗体の構造、クラス、イムノグロブリンの基準値範囲に関する技術情報など、抗体を利用した作業の基礎をご説明します。

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